TABLE OF CONTENTS
Title page
Abstract
Table of contents
Abbreviations Definitions
Glossary and Symbols
Chapter One
1.0 Introduction
1.1 Statement of research problem
1.2 Justification
1.3 Hypothesis
1.4 Specific aim and objective
Chapter Two
2.0 Literature Review
2.1 Vitamin B12
2.2 Phenyl hydrazine HCL
2.3 Anemia among women
2.3.1 Decrease haemoglobin-Oxygen affinity
2.3.2 Redistribution of blood flow
2.3.3 Increase Cardiac Output
2.4 Classification of anemia
2.4.1 Cytometric classification
2.4.2 Erythrokinetic classification
2.4.3 Reticulocyte counts
2.4.4 Serum heptaglobin concentration
2.4.5 Bone marrow biopsy
2.4.6 Biochemical classification
2.4.7 Macrocytic anemia
2.4.8 Normocytic anemia
2.4.9 Microcytic anemia
2.5.0 Dimorphic anemia
2.5.1 Heinz body
2.5.2 Hyperanemia
2.5.3 Grading of anemia
2.5.4 Causes of anemia
2.5.6 Increase destruction of Red blood cell
2.5.7 Intrinsic and Extrinsic factors
2.5.8 Mechanical trauma of Red cell
2.5.9Autoimmune haemolytic anemia
2.61 Blood loss
2.23 Treatment of anemia
2.24 Anemia and Cardiovascular system
2.25 Anemia and oxygen delivery
2.26 Anemia and haematocrit
2.27 Anemia and cardio-respiratory adjust
2.67 Anemia and cancer
2.6.8Anemia and pregnancy
2.6.9 Anemia and kidney
2.7Anemia and liver
2.8 Portal Circulation and Anemia
2.8.1 Impaired blood coagulation
2.9 Aplastic anemia
2.9.1 Alcohol, liver disease and anemia
Chapter Three
3.0 Materials and Methods
3.1.0 Collection and identification of plant material
3.1.1 Method of extraction
3.1.2 Animals
3 .2 Chemicals
3.3 Acute Toxicity studies
3.4. Experimental design
3.4.1 Induction of anemia
3.4.2 Animal groupings
3.5. Sample collection
3.6 Determination of haemoglobin concentration
3.7 Determination of the pack cell volume
3.8 Biochemical assay
3.9 Determinatoin of Bilirubin concentration
3.6 Statistical analysis
Chapter Four
4.0 Results
4.1 The role of n-butanol leaf fraction of T. Occidentalis on RBC
4.2 The role of n-butanol leaf fraction of T. Occidentalis on WBC
4.3 The role of n-butanol leaf fraction of T. Occidentalis on PCV
4.4 The role of n-butanol leaf fraction of T. Occidentalis on HB
4.5 The role of n-butanol leaf fraction of T Occidentalis on Bil conc
Chapter Five
5.0 Discussion, Conclusion and Recommendation
5.1 Discussion
5.2 Conclusion
5.3 Recommendations
REFERENCE
ABSTRACT
Anemia is a serious health problem especially in developing
countries. In the tropics, rural dwellers had resort to herbal treatment in
some cases of anemia. The main aim of this work was to ascertain the role of
n-butanol leaf fraction of Telfairia Occidentalis on some haematological
parameters in phenyl hydrazine induced anemia in wistar rats. Thirty (30) adult
Wistar rats were used for this study. All animals were assayed for
haematological parameters 1day before the onset of the experiment. The animals
were grouped into five groups of five rats each (n =5). Anemia was induced in
the Wistar rats by intraperitoneal injection of Phenyl hydrazine Hydrochloride
50mg/kg w/w in DSMO once daily for 3 days. Group i. serves as the negative
control group, received 50mg/kg of Phenylhydrazine Hydrochloride and 1ml/kg
normal saline (untreated) group ii. serve as Positive control group received
Vitamin B12 0.4ml/kg (standard haematinic) intramuscularly,while group iii, iv.
and v. received 100mg/kg, 200mg/kg and 300mg/kg body weight of n-butanol leaf
fraction of Telfairia occidentalis for 2 weeks respectively. Asessment
of RBC and WBC was carried out using the newly improved Neubauer counting
chamber, PCV using the microhaematocrit reader, Hb using the AO-Hb meter and
Bilirubin concentration using colorimetric estimation for the serum bilirubin.
There was a significant (P<0.05) increase in the level of RBC, PCV, HB and
Bilirubin concentration after treatment with the fraction as compared to the
control groups respectively. RBC (5.30±0.0217 x106/ʯL
as compared to 4.78±0.17x106/ʯL),
PCV (50.60±0.51% as compared to 37.08±0.37%), HB (16.84±0.18 g/dl as compared
to 12.56±0.13 g/dl), WBC (6.10±0.15 x109/L
as compared to 6.17±0.88x109/L)
and Bilirubin (18.16±0.08a ʯmol/L as compared to 15.54±0.21
ʯmol/L).
However, the significant increase obtained from the results of RBC ,PCV and Bilurubin
was not dose dependent while that of HB and WBC was dose dependent. In
conclusion, Intraperitoneal administration of 50mg/kg Phenylhydrazine
hydrochloride for 3days decreases the blood parameters below the pre-anemic
level (1day before induction) due to the production of reactive oxygen species.
Phytochemical screening of this leaf fraction indicates the presence of
flavonoids, saponin secoiridoid glycosides and alkaloids, these natural
antioxidants could be responsible for reversing the damaging effect of PHZ and
thus playing a modulatory role and also maintaining the integrity of the RBC.
CHAPTER ONE
1.0 INTRODUCTION
Anemia is a global public health problem affecting both developing and developed countries with major consequences for human health as well as social and economic development (WHO, 2005).
Anemia is a reduction from the normal quantity of circulating hemoglobin in the blood less than 13 g/dl for male and less than 12 g/dl for female adults (Okochi et al., 2003). It occurs at all stages of the life cycle, but is more prevalent in pregnant women and young children (Adam et al., 2005).
Hemolytic anemia is a form of anemia due to hemolysis, the abnormal breakdown of Red Blood Cells (RBCs), either in the blood vessels(intravascular hemolysis) or elsewhere in the human body (extravascular). It has numerous possible causes, ranging from relatively harmless to life-threatening (Telford et al., 2003). The general classification of hemolytic anemia is either inherited or acquired. Treatment depends on the cause and nature of the breakdown (Yamoto et al., 1998).
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