TABLE OF CONTENTS
Title Page
Certification
Dedication
Acknowledgements
Abstract
Table of Contents
List of Figures
List of Abbreviations
CHAPTER ONE: INTRODUCTION
1.1 Alcohol
1.1.1 Chemistry of alcohol
1.1.2 Alcohol production
1.1.2.1 Beer production
1.1.2.2 The production of palm wine
1.1.2.3 Production of distilled spirit
1.1.3 Absorption, distribution and metabolism of alcohol
1.1.3.1 Absorption
1.1.3.2 Distribution
1.1.3.3 Alcohol metabolism
1.1.3.3.1 Alcohol dehydrogenase pathway
1.1.3.3.2 Microsomal ethanol oxidizing system (MEOS)
1.1.4 Patterns of alcohol use and abuse
1.1.4.1 Psychological dependence
1.1.4.2 Physical dependence
1.1.4.3 Tolerance
1.1.5 Aetiology/causes of alcohol abuse
1.1.5.1 Biochemical basis
1.1.5.2 Psychosocial basis of alcohol abuse
1.1.5.3 Genetic factors
1.1.6 Effect of alcohol`
1.1.6.1 The effects of ethanol on the central nervous system
1.2 Microanatomy of the kidney
1.2.1 The nephron
1.2.2 Vulnerability of the kidney to alcohol toxicity
1.2.3 Renal function tests as indicator of alcohol induced kidney injuries
1.2.4 How alcohol-induced nephropathies affect glomerular filtration
1.2.5 Determinants of glomerular filtration
1.2.6 Creatinine clearance as a measure of glomerular filtration rate (GFR)
1.3 Serum electrolyte and alcohol-induced renal dysfunctions
1.4 Alcohol induced pathological proteinuria
1.5 Urine specific gravity as an index of alcohol-induced renal dysfunction
1.6 Alteration in plasma urea concentration as a pointing indicator of renal dysfunction under the nephrotoxic assault of chronic and excessive alcohol abuse
1.7 Microanatomy of the human liver
1.8 The liver and alcohol metabolising system
1.9 The nature and metabolic roles of some alcohol metabolizing enzymes
1.9.1 Alcohol dehydrogenase
1.9.2 Cytochrome P450
1.9.3 Aldehyde dehydrogenase
1.10 Some alcohol-induced liver problems
1.10.1 Ethanol-induced liver dysfunctions
1.11 Alcoholic liver diseases
1.11.1 Hepatic steatosis (fatty liver)
1.11.2 Alcohol hepatitis
1.11.3 Alcoholic cirrhosis
1.12 Alcohol-induced liver degeneration and intracellular accumulation
1.13 Bilirubin metabolism and alcohol-induced liver dysfunction
1.14 Diagnosis of alcohol-induced liver problems
1.14.1 Enzymes of diagnostic utility in alcohol induced liver dysfunction
1.14.1.1 Aminotransferases
1.14.1.2 Alkaline phosphatase (ALP)
1.15 Justification of the study
1.16 Aim and objectives of the study
1.16.1 Aim of the study
1.16.2 Objectives of the study
CHAPTER TWO: MATERIALS AND METHODS
2.1 Materials
2.1.1 Subjects used
2.1.2 Location/Zone of Study
2.1.3 Chemicals/Reagents/Samples
2.1.4 Instruments/Equipment
2.2 Methods
2.2.1 Experimental design
2.2.2 Collection of 24-hour urine sample
2.2.3 Collection of blood specimens
2.2.4 Experimental analyses
2.2.4.1 Determination of urine total protein concentration
2.2.4.2 Measurement of urine specific gravity
2.2.4.3 Determination of urea concentration
2.2.4.4 Determination of creatinine concentration
2.2.4.5 Determination of serum potassium ion concentration
2.2.4.6 Determination of serum sodium ion concentration
2.2.4.7 Determination of direct bilirubin concentration
2.2.4.8 Assay of alkaline phosphatase (ALP) activity
2.2.4.9 Assay of aspartate aminotransferase (AST) activity
2.2.4.10 Assay of alanine aminotransferase (ALT) activity
2.2.4.11 Assay of gamma-glutamyltransferase activity
2.2.4.12 Measurement of body mass index
2.2.4.13 Measurement of blood pressure
2.3 Statistical analysis
CHAPTER THREE: RESULTS
3.1 Effect of alcohol consumption on urine total protein concentration in human subjects
3.2 Effect of alcohol consumption on urine specific gravity in human subjects
3.3 Effect of alcohol consumption on serum urea concentration in human subjects
3.4 Effect of alcohol consumption on serum creatinine concentration in human subjects
3.5 Effect of alcohol consumption on urine creatinine concentration in human subjects
3.6 Effect of alcohol consumption on creatinine clearance in human subjects
3.7 Effect of alcohol consumption on serum potassium concentration in human subjects
3.8 Effect of alcohol consumption on serum sodium concentration in human subjects
3.9 Effect of alcohol consumption on serum total bilirubin concentration in human subjects
3.10 Effect of alcohol consumption on serum alkaline phosphatase activity in human subjects
3.11 Effect of alcohol consumption on serum aspartate aminotransferase activity in human subjects
3.12 Effect of alcohol consumption on serum alanine aminotransferase activity in human subjects
3.13 Effect of alcohol consumption on gamma glutamyltransferase activities in humans subjects
3.14 Effect of alcohol consumption on body mass index in human subjects
3.15 Effect of alcohol consumption on blood pressure in human subjects
CHAPTER FOUR: DISCUSSION
4.1 Discussion
4.2 Conclusion
4.3 Suggestions for Further Research
REFERENCES
APPENDICES
ABSTRACT
This work was aimed at finding
the effects of alcohol on some biochemical parameters. A total of one hundred
and eighty (180) apparently healthy, non-hypertensive male alcoholics were used
for the study. Forty (40) non-consumers of alcohol were used as control. The
activity of alanine aminotransferase (ALT) in the control was 10.50±2.00 IU/L
while it was 16.50±1.50 IU/L; 17.50±2.00 IU/L and 18.31±2.00 IU/L in alcoholics
who showed preference for palmwine, beer and distilled spirit respectively.
Also, the activity of aspartate aminotransferase (AST) in the control was
9.51±0.35 IU/L while it was 18.44±0.40 IU/L, 19.21±0.19 IU/L, 20.32±0.64 IU/L i
n alcoholics who showed preference for palmwine, beer and distilled spirit
respectively. The ALT and AST activities of alcoholic subjects who showed
preference for distilled spirit was significantly higher (p < 0.05) than
those who showed preference for palmwine and beer. The activities of alcoholics
who showed preference for palmwine was the lowest. Furthermore, the serum total
bilirubin concentration of the alcoholics was significantly higher (p <
0.05) compared with the control. The serum total bilirubin concentrations were
18.65±2.10
μmol/l, 19.40±1.50 μmol/l and 22.75±1.60 μmol/l for
alcoholics who showed preference for palmwine, beer and distilled spirit
respectively. The serum total bilirubin of the control was 8.30 ± 2.00 μmol/l.
The alkaline phosphatase (ALP) activity of the alcoholic subjects was
significantly higher (p<0.05) compared with the control. The ALP activity of
the control was 61.50 ± 30.00 IU/L while the ALP activity was 174.20±2.50 IU/L,
175.10±1.50 IU/L and 177.40±1.00 IU/L in the three categories of alcoholics who
showed preference for palmwine, beer and distilled spirit respectively.
Moreover, the urine total protein concentration of the alcoholics was
significantly higher (p<0.05) compared with the control. Alcoholics who
showed preference for distilled spirit had urine total protein of 153.96±0.43
mg/dl followed by alcoholics who showed preference for beer and palmwine who
had urine total protein of 152.74±0.42 mg/dl and 151.34±0.60 mg/dl respectivel y.
The urine total protein of the control was 56.40±0.40 mg/dl. Furthermore, the
urine specific gravity, serum urea and creatinine of the alcoholics were
significantly higher (p < 0.05) compared with the control. However, the
plasma sodium, potassium and creatinine clearance of the alcoholics were
significantly lower (p < 0.05) compared with the control. The body mass
index (BMI) of the three groups of alcoholics fell within the range of 18.50 to
24.90. The blood pressure of both the alcoholic and control subjects were
normal (below 140/90 mmHg). This work therefore shows that chronic alcohol use
could induce both hepatic and renal dysfunctions in the alcoholics which
manifested in form of adverse variations in some biochemical parameters of
prognostic and diagnostic utility.
CHAPTER ONE
INTRODUCTION
Generally, alcohol designates a class of compounds that are hydroxyl derivatives of aliphatic hydrocarbons. However, in this study, the term alcohol used without additional qualifications refers specifically to ethanol. A variety of alcoholic beverages have been consumed by man in the continuing search for euphoria producing stimuli. Among some people, alcohol enjoys a high status as a social lubricant that relieves tension, gives self confidence to the inadequate, blurs the appreciation of uncomfortable realities and serves as an escape from environmental and emotional stress.
Alcohol has been loved and hated at different times by different people. Alcohol has been celebrated as healthful especially to the heart (red wine) and most pleasant to the taste buds; and then dismissed as “demon’s rum” and “devil in solution” depen ding on the prevalent view.
In spite of the apparent divergent and sometimes conflicting opinions about alcohol, the consensus shared by drinkers and non drinkers alike is that excessive and chronic consumption of alcohol is a disorder. Like any other chronic disorder, it develops insidiously but follows a predictable course. The first or pre-alcoholic symptomatic phase begins with the use of alcohol to relieve tensions. The second (or prodromal) phase is marked by a range of behaviours including preoccupation with alcohol, surreptitious drinking and loss of memory (Hock et al., 1992). In the third (or crucial) phase, the individual loses control over his drinking. This loss of control is the beginning of the disease process of addiction. The individual starts drinking early in the morning and stays up drinking till late in the night. Impairment in biochemical activities becomes manifest as the organs of the alcoholic begin to deteriorate. Other medical problems develop by the time the alcoholic gets into the final (chronic phase). Prolonged intoxications become the rule. Alcoholic psychosis develops, thinking is impaired, and fear and tremors become persistent (Klemin and Sherry, 1981). A previously responsible individual may be transformed into an inebriate – s tereotype alcoholic.
Fear-instilling but thought- provoking terms such as the “coming epidemic”, a “miserable trap”, have b een used to show concern for the potential hazard of widespread alcoholism.
In its 1978 revision of the international classification of diseases, the World Health Organization defined alcoholism as “a state, psychic and usually also physical, resulting from taking alcohol, characterised by behavioural and other responses that always include a compulsion to take alcohol on...
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